Journal: Frontiers in Physiology

Article Title: High Glucose Attenuates Cardioprotective Effects of Glucagon-Like Peptide-1 Through Induction of Mitochondria Dysfunction via Inhibition of β-Arrestin-Signaling

doi: 10.3389/fphys.2021.648399

Figure Lengend Snippet: Catalog numbers of commercially available kits and agents.

Article Snippet: ROS assay kit , Abcam, United Kingdom , ab113851.

Techniques: TUNEL Assay, Caspase-3 Assay, ROS Assay, CCK-8 Assay

Journal: Marine drugs

Article Title: Sulfated Triterpene Glycosides from the Far Eastern Sea Cucumber Cucumaria djakonovi : Djakonoviosides C 1 , D 1 , E 1 , and F 1 ; Cytotoxicity against Human Breast Cancer Cell Lines; Quantitative Structure-Activity Relationships.

doi: 10.3390/md21120602

Figure Lengend Snippet: Figure 4. Migration of MDA-MB-231 and MCF-7 cells into wound areas observed with MIB-2-FL fluorescence microscope at 10-fold magnification: (a) MDA-MB-231 cells 0 and 24 h after treatment with 0.5, 1.0, and 2.0 µM of djakonovioside C1 (1); (b) MDA-MB-231 cells 0 and 24 h after treatment with 0.25, 0.5, and 1.0 µM of cucumarioside A2-5 (6); (c) MDA-MB-231 cells 0 and 24 h after treatment with 0.5, 1.0, and 2.0 µM of djakonovioside E1 (3); (d,e) MCF-7 cells 0 and 72 h after treatment with 0.5, 1.0, and 2.0 µM of djakonovioside E1 (3). Cells were stained with the fluorescent dye CFDA SE. Cell migration into wound areas processed by ImageJ 1.52 software. Data are presented as means ± SEM. * p value ≤0.05 considered significant.

Article Snippet: Cell debris and floating cells were deleted by washing twice with PBS; 10 μM of 5 mM initial solution of CFDA SE ((5,6)- carboxyfluorescein succinimidyl ester) (LumiTrace CFDA SE kit, Lumiprobe, Moscow, Russia) in DMSO was dissolved in PBS and added to cells for 5 min at 37 ◦C; after washing twice with PBS, the fresh culture medium was added.

Techniques: Migration, Microscopy, Staining, Software